Enhanced bone marrow derived mesenchymal stem cell differentiation when isolated and expanded with human platelet rich plasma and differentiation media is supplemented with vitamin D

Gauri Thapa; Alice Tirnoveanu; Athina Mylona; Richard Webb

Enhanced bone marrow derived mesenchymal stem cell differentiation when isolated and expanded with human platelet rich plasma and differentiation media is supplemented with vitamin D

Gauri Thapa
, Alice Tirnoveanu
, Athina Mylona
, Richard Webb

Research output: Contribution to journal › Article › peer-review

Abstract

Vitamin D3 is well known to be involved in bone formation during foetal development and has been shown to be actively involved from gestational day 13 during foetal rat development [1]. Vitamin D3 and its associated enzyme 1-alpha-hydroxylase a member of the cytochrome P450 super family and encoded for by the gene CYP27B1. In this study we supplemented osteogenic media with vitamin D at a concentration of 10-4M and differentiated for 21 days with samples analysed by means of quantitative alizarin red assay and qPCR for the bone markers RUNX2, ALPL and HPRT1

Original language	English
Journal	eCM Periodicals & Conferences
Publication status	Published - 20 Oct 2020

Keywords

Cell therapy
Developmental biology

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abstract = "Vitamin D3 is well known to be involved in bone formation during foetal development and has been shown to be actively involved from gestational day 13 during foetal rat development [1]. Vitamin D3 and its associated enzyme 1-alpha-hydroxylase a member of the cytochrome P450 super family and encoded for by the gene CYP27B1. In this study we supplemented osteogenic media with vitamin D at a concentration of 10-4M and differentiated for 21 days with samples analysed by means of quantitative alizarin red assay and qPCR for the bone markers RUNX2, ALPL and HPRT1",

keywords = "Cell therapy, Developmental biology",

author = "Gauri Thapa and Alice Tirnoveanu and Athina Mylona and Richard Webb",

year = "2020",

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journal = "eCM Periodicals \& Conferences",

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T1 - Enhanced bone marrow derived mesenchymal stem cell differentiation when isolated and expanded with human platelet rich plasma and differentiation media is supplemented with vitamin D

AU - Thapa, Gauri

AU - Tirnoveanu, Alice

AU - Mylona, Athina

AU - Webb, Richard

PY - 2020/10/20

Y1 - 2020/10/20

N2 - Vitamin D3 is well known to be involved in bone formation during foetal development and has been shown to be actively involved from gestational day 13 during foetal rat development [1]. Vitamin D3 and its associated enzyme 1-alpha-hydroxylase a member of the cytochrome P450 super family and encoded for by the gene CYP27B1. In this study we supplemented osteogenic media with vitamin D at a concentration of 10-4M and differentiated for 21 days with samples analysed by means of quantitative alizarin red assay and qPCR for the bone markers RUNX2, ALPL and HPRT1

AB - Vitamin D3 is well known to be involved in bone formation during foetal development and has been shown to be actively involved from gestational day 13 during foetal rat development [1]. Vitamin D3 and its associated enzyme 1-alpha-hydroxylase a member of the cytochrome P450 super family and encoded for by the gene CYP27B1. In this study we supplemented osteogenic media with vitamin D at a concentration of 10-4M and differentiated for 21 days with samples analysed by means of quantitative alizarin red assay and qPCR for the bone markers RUNX2, ALPL and HPRT1

KW - Cell therapy

KW - Developmental biology

UR - https://www.ecmconferences.org/abstracts/2020/Collection1/c1.html

M3 - Article

SN - 2522-235X

JO - eCM Periodicals & Conferences

JF - eCM Periodicals & Conferences

ER -

Enhanced bone marrow derived mesenchymal stem cell differentiation when isolated and expanded with human platelet rich plasma and differentiation media is supplemented with vitamin D

Abstract

Keywords

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