Multicentre study on the reproducibility of MALDI-TOF MS for nontuberculous mycobacteria identification

D. Rodriguez-Temporal; F. Alcaide; I. Mareković; James O'Connor; R. Gorton; J. van Ingen; A. Van den Bossche; G. Héry-Arnaud; C. Beauruelle; D. Orth-Höller; J.-J. Palacios-Gutiérrez; G. Tudó; G. Bou; P.-J. Ceyssens; M. Garrigó; J. González-Martin; G. Greub; J. Hrabak; A. Ingebretsen; M.C. Mediavilla-Gradolph; M. Oviaño; B. Palop; A.B. Pranada; L. Quiroga; M.J. Ruiz-Serrano; B. Rodríguez-Sánchez

doi:10.1038/s41598-022-05315-7

Multicentre study on the reproducibility of MALDI-TOF MS for nontuberculous mycobacteria identification

D. Rodriguez-Temporal
, F. Alcaide
, I. Mareković
, James O'Connor
, R. Gorton
, J. van Ingen
, A. Van den Bossche
, G. Héry-Arnaud
, C. Beauruelle
, D. Orth-Höller
, J.-J. Palacios-Gutiérrez
, G. Tudó
, G. Bou
, P.-J. Ceyssens
, M. Garrigó
, J. González-Martin
, G. Greub
, J. Hrabak
, A. Ingebretsen
, M.C. Mediavilla-Gradolph

M. Oviaño, B. Palop, A.B. Pranada, L. Quiroga, M.J. Ruiz-Serrano, B. Rodríguez-Sánchez

Natural Sciences

Research output: Contribution to journal › Article › peer-review

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Abstract

The ability of MALDI-TOF for the identification of nontuberculous mycobacteria (NTM) has improved recently thanks to updated databases and optimized protein extraction procedures. Few multicentre studies on the reproducibility of MALDI-TOF have been performed so far, none on mycobacteria. The aim of this study was to evaluate the reproducibility of MALDI-TOF for the identification of NTM in 15 laboratories in 9 European countries. A total of 98 NTM clinical isolates were grown on Löwenstein-Jensen. Biomass was collected in tubes with water and ethanol, anonymized and sent out to the 15 participating laboratories. Isolates were identified using MALDI Biotyper (Bruker Daltonics). Up to 1330 MALDI-TOF identifications were collected in the study. A score ≥ 1.6 was obtained for 100% of isolates in 5 laboratories (68.2–98.6% in the other). Species-level identification provided by MALDI-TOF was 100% correct in 8 centres and 100% correct to complex-level in 12 laboratories. In most cases, the misidentifications obtained were associated with closely related species. The variability observed for a few isolates could be due to variations in the protein extraction procedure or to MALDI-TOF system status in each centre. In conclusion, MALDI-TOF showed to be a highly reproducible method and suitable for its implementation for NTM identification.

Original language	English
Article number	1237
Journal	Scientific Reports
DOIs	https://doi.org/10.1038/s41598-022-05315-7
Publication status	Published - 24 Jan 2022

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 3 Good Health and Well-being

Keywords

Clinical microbiology
Infectious-disease diagnostics

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Cite this

Rodriguez-Temporal, D., Alcaide, F., Mareković, I., O'Connor, J., Gorton, R., van Ingen, J., Van den Bossche, A., Héry-Arnaud, G., Beauruelle, C., Orth-Höller, D., Palacios-Gutiérrez, J.-J., Tudó, G., Bou, G., Ceyssens, P.-J., Garrigó, M., González-Martin, J., Greub, G., Hrabak, J., Ingebretsen, A., ... Rodríguez-Sánchez, B. (2022). Multicentre study on the reproducibility of MALDI-TOF MS for nontuberculous mycobacteria identification. Scientific Reports, Article 1237. https://doi.org/10.1038/s41598-022-05315-7

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title = "Multicentre study on the reproducibility of MALDI-TOF MS for nontuberculous mycobacteria identification",

abstract = "The ability of MALDI-TOF for the identification of nontuberculous mycobacteria (NTM) has improved recently thanks to updated databases and optimized protein extraction procedures. Few multicentre studies on the reproducibility of MALDI-TOF have been performed so far, none on mycobacteria. The aim of this study was to evaluate the reproducibility of MALDI-TOF for the identification of NTM in 15 laboratories in 9 European countries. A total of 98 NTM clinical isolates were grown on L{\"o}wenstein-Jensen. Biomass was collected in tubes with water and ethanol, anonymized and sent out to the 15 participating laboratories. Isolates were identified using MALDI Biotyper (Bruker Daltonics). Up to 1330 MALDI-TOF identifications were collected in the study. A score ≥ 1.6 was obtained for 100\% of isolates in 5 laboratories (68.2{\textendash}98.6\% in the other). Species-level identification provided by MALDI-TOF was 100\% correct in 8 centres and 100\% correct to complex-level in 12 laboratories. In most cases, the misidentifications obtained were associated with closely related species. The variability observed for a few isolates could be due to variations in the protein extraction procedure or to MALDI-TOF system status in each centre. In conclusion, MALDI-TOF showed to be a highly reproducible method and suitable for its implementation for NTM identification.",

keywords = "Clinical microbiology, Infectious-disease diagnostics",

author = "D. Rodriguez-Temporal and F. Alcaide and I. Marekovi{\'c} and James O'Connor and R. Gorton and \{van Ingen\}, J. and \{Van den Bossche\}, A. and G. H{\'e}ry-Arnaud and C. Beauruelle and D. Orth-H{\"o}ller and J.-J. Palacios-Guti{\'e}rrez and G. Tud{\'o} and G. Bou and P.-J. Ceyssens and M. Garrig{\'o} and J. Gonz{\'a}lez-Martin and G. Greub and J. Hrabak and A. Ingebretsen and M.C. Mediavilla-Gradolph and M. Ovia{\~n}o and B. Palop and A.B. Pranada and L. Quiroga and M.J. Ruiz-Serrano and B. Rodr{\'i}guez-S{\'a}nchez",

year = "2022",

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day = "24",

doi = "10.1038/s41598-022-05315-7",

language = "English",

journal = "Scientific Reports",

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Rodriguez-Temporal, D, Alcaide, F, Mareković, I, O'Connor, J, Gorton, R, van Ingen, J, Van den Bossche, A, Héry-Arnaud, G, Beauruelle, C, Orth-Höller, D, Palacios-Gutiérrez, J-J, Tudó, G, Bou, G, Ceyssens, P-J, Garrigó, M, González-Martin, J, Greub, G, Hrabak, J, Ingebretsen, A, Mediavilla-Gradolph, MC, Oviaño, M, Palop, B, Pranada, AB, Quiroga, L, Ruiz-Serrano, MJ & Rodríguez-Sánchez, B 2022, 'Multicentre study on the reproducibility of MALDI-TOF MS for nontuberculous mycobacteria identification', Scientific Reports. https://doi.org/10.1038/s41598-022-05315-7

TY - JOUR

T1 - Multicentre study on the reproducibility of MALDI-TOF MS for nontuberculous mycobacteria identification

AU - Rodriguez-Temporal, D.

AU - Alcaide, F.

AU - Mareković, I.

AU - O'Connor, James

AU - Gorton, R.

AU - van Ingen, J.

AU - Van den Bossche, A.

AU - Héry-Arnaud, G.

AU - Beauruelle, C.

AU - Orth-Höller, D.

AU - Palacios-Gutiérrez, J.-J.

AU - Tudó, G.

AU - Bou, G.

AU - Ceyssens, P.-J.

AU - Garrigó, M.

AU - González-Martin, J.

AU - Greub, G.

AU - Hrabak, J.

AU - Ingebretsen, A.

AU - Mediavilla-Gradolph, M.C.

AU - Oviaño, M.

AU - Palop, B.

AU - Pranada, A.B.

AU - Quiroga, L.

AU - Ruiz-Serrano, M.J.

AU - Rodríguez-Sánchez, B.

PY - 2022/1/24

Y1 - 2022/1/24

N2 - The ability of MALDI-TOF for the identification of nontuberculous mycobacteria (NTM) has improved recently thanks to updated databases and optimized protein extraction procedures. Few multicentre studies on the reproducibility of MALDI-TOF have been performed so far, none on mycobacteria. The aim of this study was to evaluate the reproducibility of MALDI-TOF for the identification of NTM in 15 laboratories in 9 European countries. A total of 98 NTM clinical isolates were grown on Löwenstein-Jensen. Biomass was collected in tubes with water and ethanol, anonymized and sent out to the 15 participating laboratories. Isolates were identified using MALDI Biotyper (Bruker Daltonics). Up to 1330 MALDI-TOF identifications were collected in the study. A score ≥ 1.6 was obtained for 100% of isolates in 5 laboratories (68.2–98.6% in the other). Species-level identification provided by MALDI-TOF was 100% correct in 8 centres and 100% correct to complex-level in 12 laboratories. In most cases, the misidentifications obtained were associated with closely related species. The variability observed for a few isolates could be due to variations in the protein extraction procedure or to MALDI-TOF system status in each centre. In conclusion, MALDI-TOF showed to be a highly reproducible method and suitable for its implementation for NTM identification.

AB - The ability of MALDI-TOF for the identification of nontuberculous mycobacteria (NTM) has improved recently thanks to updated databases and optimized protein extraction procedures. Few multicentre studies on the reproducibility of MALDI-TOF have been performed so far, none on mycobacteria. The aim of this study was to evaluate the reproducibility of MALDI-TOF for the identification of NTM in 15 laboratories in 9 European countries. A total of 98 NTM clinical isolates were grown on Löwenstein-Jensen. Biomass was collected in tubes with water and ethanol, anonymized and sent out to the 15 participating laboratories. Isolates were identified using MALDI Biotyper (Bruker Daltonics). Up to 1330 MALDI-TOF identifications were collected in the study. A score ≥ 1.6 was obtained for 100% of isolates in 5 laboratories (68.2–98.6% in the other). Species-level identification provided by MALDI-TOF was 100% correct in 8 centres and 100% correct to complex-level in 12 laboratories. In most cases, the misidentifications obtained were associated with closely related species. The variability observed for a few isolates could be due to variations in the protein extraction procedure or to MALDI-TOF system status in each centre. In conclusion, MALDI-TOF showed to be a highly reproducible method and suitable for its implementation for NTM identification.

KW - Clinical microbiology

KW - Infectious-disease diagnostics

U2 - 10.1038/s41598-022-05315-7

DO - 10.1038/s41598-022-05315-7

M3 - Article

SN - 2045-2322

JO - Scientific Reports

JF - Scientific Reports

M1 - 1237

ER -

Multicentre study on the reproducibility of MALDI-TOF MS for nontuberculous mycobacteria identification

Abstract

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Keywords

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